Figure 4
From: The PagN protein of Salmonella enterica serovar Typhimurium is an adhesin and invasin
Characterization of pagN mutant strain ML6. (A) Colonies of strains SL1344 and ML6 were boiled and used as a template in a PCR reaction with primers PagNF2 and PagNR2 to amplify the pagN ORF. MW indicates molecular size markers. Lane 1 ML6, lane 2 SL1344. (B) Sarkosyl-extracted outer membrane proteins were electrophoresed through a 15% gel. Proteins were transferred to PVDF membrane and probed with an anti-PagN antibody. The position of PagN is arrowed. When expressed from a multicopy plasmid PagN was found to migrate as a doublet in common with the Hek protein. Lane 1, SL1344; Lane 2, ML6; Lane 3, ML6 with pBR322; Lane 4, ML6 with pML10.