Table 2 Strains and plasmids used in this study.

Strains

E2348/69

Wild-type EPEC strain

EPEC (O127:H6) isolated from an infant with gastroenteritis

[27]

MC4100

Wild-type E. coli K-12 lab strain

F^- araD139 Δ(argF-lac)U169 rpsL150(Str^R) relA1 flbB5301 deoC1 ptsF25 rbsR

[28]

TOP10 cells

Commercially available competent cells for cloning

F^- mcrA Δ(mrr-hsdRMS-mcfBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu) 7697 galU galK rpsL endA1 nupG (Str^R)

Invitrogen Canada Inc.

TAM1 λpir

Commercially available competent cells of E. coli λlysogen that provide all trans acting and mobilization factors required for the replication and mobilization of λPi dependent plasmids

mcrA Δ(mrr-hsdRMS-mcfBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu) 7697 galU galK rpsL endA1 nupG λpir

Active Motif

SM10 λpir

E. coli SM10 λlysogen that provide all trans acting and mobilization factors required for the replication and mobilization of λPi dependent plasmids

[29]

NH1

TOP10 (pUC19 ΔhsdR)

This study

NH3

SM10 λpir (pCVD442 ΔhsdR)

This study

NH4

E2348/69 ΔhsdR

This study

LP69

MC4100 nadA::Tn10

Lab stock

Plasmids

pACYC184

5 kb low copy number cloning vector

[16]

pUC19

Cloning vector

Invitrogen Canada Inc.

pUC19 ΔhsdR

pUC19 carrying ΔhsdR construct

This study

pCVD442

Cloning vector requiring λ Pi protein to replicate, carries sacB for negative selection (Amp^R)

[10]

pCVD442 ΔhsdR

pCVD442 carrying ΔhsdR construct

This study

pJW15

Broad host range promoterless lux reporter plasmid

[3, 13]

pLAFR1

Low copy number, broad host range plasmid

[17]

pNLP10

Low copy number lux reporter plasmid, pSC101 origin

[3, 13]