From: Development of methods for the genetic manipulation of Flavobacterium columnare
Strain or plasmid | Genotype or description | Source or reference |
---|---|---|
Bacterial Strains | Â | Â |
   E. coli |  |  |
S17-1 | hsdR17 (rk- mk-)recA RP4-2(Tcr::Mu-Kmr::Tn7 Strr) | Â |
TOP10 | F- mcrA Δ(mrr-hsdRMA-mcrBC) φ80lacΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
   F. columnare |  |  |
C#2 | Wild Type | [13] |
AL-203-94 | Wild Type | [13] |
Fc14-56 | Wild Type | [26] |
94-060 | Wild Type | [26] |
1191-B | Wild Type | [26] |
94-078 | Wild Type | [26] |
94-081 | Wild Type | [26] |
90-059 | Wild Type | [26] |
L90-659 | Wild Type | [26] |
92-002 | Wild Type | [26] |
C91-20 | Wild Type | [26] |
FcAS44 | gldJ knockout mutant of C#2 | This Study |
Plasmids | Â | Â |
   pAMSTA39 | PCR cloning vector with promoter-less gfp; Apr Kmr | This Study |
   pAS29 | Promoter-less gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr) | This Study |
   pAS36 | Pmap-gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr) | This Study |
   pAS42 | 1400-bp fragment of gldJ in pCP29; Apr (Emr, Cfr) | This Study |
   pAS43 | PompA-gfp containing E. coli-Flavobacterium shuttle vector; Apr (Emr, Cfr) | This Study |
   pCE320 | gfp-containing E. coli-Borrelia burgdorferi shuttle vector; Apr | [46] |
   pCR4-TOPO | PCR cloning vector; Apr Kmr | Invitrogen |
   pCP11 | E. coli – Flavobacterium shuttle plasmid; Apr (Emr) | [21] |
   pCP29 | E. coli – Flavobacterium shuttle plasmid; Apr (Cfr Emr) | [37] |
   pEP4351 | λpir dependent R6K oriV; RP4 oriT; Cmr Tcr (Emr); Tn4351 mutagenesis vector | [53] |
   pCR4-TOPO | PCR cloning vector; Apr Kmr | Invitrogen |