Skip to main content
Figure 1 | BMC Microbiology

Figure 1

From: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples

Figure 1

Sensitivity of PCR for detection of minimum number of E. moshkovskii (EM), E. histolytica (EH) and E. dispar (ED) cells. 0.05 gm of negative control stool specimen seeded with serially diluted Entamoeba cells corresponding to 106 cells (lane 1), 105 cells (lane 2), 104 cells (lane 3), 103 cells (lane 4), 102 cells (lane 5), and 10 cells (lane 6) were subjected to DNA extraction followed by PCR amplification. Amplified products were analyzed by agarose gel electrophoresis. The sizes of the amplification products are indicated on the left (in base pairs). Lanes 7, negative control (PCR without DNA)

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us