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Table 1 Regulatory dependence of type III secreted effectors under SPI-1 inducing conditions.

From: Systematic analysis of the regulation of type three secreted effectors in Salmonella enterica serovar Typhimurium

  

A

B

C

D

E

F

G

H

I

  

wild type

MM+ pWSK29

MM sprB+

MM hilC+

MM hilD+

MM hilA +

MM invF+ sicA +

MM ssrB+

MM sirA +

1

sopA-lacZ

7685 ± 1026

23 ± 1

1 ± 2

0 ± 2

1 ± 2

9 ± 3

4982 ± 625

9 ± 3

1 ± 1

2

sopB-lacZ

10981 ± 922

148 ± 11

85 ± 2

75 ± 8

82 ± 4

199 ± 15

56160 ± 4842

3095 ± 1711

113 ± 8

3

sopE2-lacZ

14251 ± 4224

1872 ± 252

2425 ± 748

2477 ± 281

1093 ± 391

3735 ± 65

31415 ± 1109

3963 ± 90

2574 ± 69

4

slrP-lacZ

385 ± 44

305 ± 5

3511 ± 349

295 ± 164

448 ± 78

403 ± 87

1282 ± 138

308 ± 39

621 ± 23

5

sspHl-lacZ

796 ± 196

689 ± 26

697 ± 165

668 ± 98

949 ± 103

960 ± 146

2906 ± 585

827 ± 165

666 ± 67

6

sspH2-lacZ

360 ± 34

326 ± 38

412 ± 128

225 ± 16

389 ± 71

453 ± 90

359 ± 24

15685 ± 1481

296 ± 11

7

sifA-lacZ

21664 ± 538

10321 ± 719

9970 ± 528

9232 ± 665

9611 ± 887

10176 ± 817

10329 ± 1153

14085 ± 429

16738 ± 1612

8

sifB-lacZ

65 ± 2

939 ± 81

1393 ± 849

19 ± 5

1537 ± 470

809 ± 150

30 ± 6

14334 ± 2904

25 ± 6

9

sseI-lacZ

70 ± 4

60 ± 10

69 ± 22

32 ± 2

43 ± 5

61 ± 15

47 ± 10

5154 ± 1291

60 ± 15

10

sseG-lacZ

1931 ± 36

2046 ± 440

1153 ± 257

3001 ± 297

2083 ± 442

2872 ± 314

2444 ± 554

25454 ± 314

919 ± 73

  1. The lac fusion being measured is listed in the left column. The strain background is listed along the top. Each strain harbors a lac fusion to an effector and a plasmid encoding a regulator or the vector control. The values in bold are those showing greater than 3-fold differences compared to the MM + pWSK29 strain that are discussed in the text. Each value represents the mean of at least three experiments ± standard deviation. MM, multi-regulator mutant [Δ(avrA-invH)1 ssrB::cat sirA4::hyg].
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BMC Microbiology

ISSN: 1471-2180

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