Table 3 Oligonucleotides used in this study
Oligonucleotide | Sequence (5'-3')* | Restriction sites |
|---|---|---|
Fha1F | 5'-CTCATCATCGCCAACCCCAACGG -3' | - |
Fha1R | 5'-AGCTGGCGCACGCCCAGGCCTG -3' | - |
Fha2F | 5'-CCCAAGCCCAAGCCCAAGCCCAAGGCC -3' | - |
Fha2R | 5'-ATAGAAGACCCGGTAGTTCT -3' | - |
FhaBamHI | 5'-CCTCGGAGGATCC CCTCCATCGA -3' | BamHI |
FhaHindIII | 5'-TACTTTGCTGAAGCTT AAACGATAG -3' | HindIII |
Fhagfp1 | 5'-CCTCGGAGGATCC CCTCCATCGA -3' | BamHI |
Fhagfp2 | 5'-ACAACGAGAGGATCC GCAGCAA -3' | BamHI |
Fhagfp3 | 5'-CAAAAGGGGATCC ACGGGGCAA -3' | BamHI |
Fhagfp4 | 5'-AGGGTGCGAGGATCC TGACACA -3' | BamHI |
Fhagfp6 | 5'-AAGTGTTGGGATCC GTAGTGTCT -3' | BamHI |
Fhagfp7 | 5'-AACGATCTAGA TCCGCGCTGCCC -3' | XbaI |
FhaGR1 | 5'-GACTATCCTGACACATTGAGGAG -3' | - |
FhaGR2 | 5'-CTACATGTAAGTAGGGCCCTGTG -3' | - |
Gfp1 | 5'-CAAGAATTGGGACAACTCCAGT -3' | - |