Figure 2
From: Human inflammatory bowel disease does not associate with Lawsonia intracellularis infection
Quantification of 16SI-amplified products by Q-PCR analysis of intestinal DNA preparation. Bacterial DNA was prepared from intestinal specimens obtained from IBD patients as described in Material and Methods, pre-amplified for 10 cycles, diluted 1:100 and further amplified in LightCycler using primers recognizing bacterial 16S subunit (16SI). Data are presented as an absolute number of copies per μl of input DNA (A) or as relative bacterial load calculated as ratio of 16SI-amplicons to β-actin amplicons (B). Note that this calculation is not applicable in the case of porcine fecal samples.