Figure 5
From: A novel flow cytometric assay for measurement of In Vivo pulmonary neutrophil phagocytosis
Stability of CFSE Labeling of Pneumococci. To ensure that CFSE did not leak out of labeled pneumococci when they were in the rats' lungs, CFSE labeled pneumococci were incubated with fresh lavage fluid. After centrifugation to remove the bacteria the supernatant was incubated with lavage cells. Lavage cells before incubation with the supernatant (gray shading) and after (black line).