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Table 5 Regulation of E. coli and Y. pestis dppA::lacZ translational gene fusions by the Y. pestis gcvB gene. Cells were grown in LB (E. coli) or in HIB (Y. pestis) at 37°C to an OD600 ~0.5 and assayed for β-galactosidase activity [16]. Activity is expressed in Miller units. The parent strains KIM6 and KIM6ΔgcvB grown in HIB at 37°C showed <5 units of β-galactosidase activity.

From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules

Lysogen

Relevant genotype

β-Galactosidase activity

GS162λdppAEc::lacZ

WT

103 ± 24

GS1144λdppAEc::lacZ

ΔgcvB

554 ± 81

GS1144λdppAEc::lacZ[pgcvBYp-p322]

ΔgcvB/gcvBYp

154 ± 32

KIM6[pdppAYp::lacZ]

WT

62 ± 13

KIM6ΔgcvB[pdppAYp::lacZ]

ΔgcvB

455 ± 7