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Table 2 Consequences of selective manipulation of MSggN media composition on surface colony formation of the hag null strain. This data shows how systematically lowering concentrations of macronutrients and micronutrients affects the growth pattern on MSggN agarose plates. In each case 5 mM KCl was present, except where KCl was varied, and plates were inoculated in triplicate to reveal the surface colony pattern (film or tendrils) after 24 h growth at 37°C.

From: A defined medium to investigate sliding motility in a Bacillus subtilis flagella-less mutant

Media component

1X conc.

0.1X conc.

0.01X conc.

Macronutrients

   

54 mM Glycerol

Film

Tendril

Tendril

30 mM Na glutamate

Film

Tendril

Weak growth

5 mM NaH2PO4

Film

Tendril

Tendril

Micronutrients

   

5 mM KCl

Film

Tendril

Tendril

2 mM MgCl2

Film

Film

Tendrila

700 μM CaCl2

Film

Film

Filmb

50 μM MnSO4

Film

Film

Filmc

50 μM FeSO4

Film

Film

Filmd

1 μM ZnSO4

Film

Film

Filmb

  1. aTendrils were also formed at the 10-3 to 10-5 dilutions.
  2. bFor these micronutrients, films were also formed at the 10-3 to 10-5 dilutions.
  3. cFilms were formed at the 10-3 dilution and tendrils were formed at the 10-4 and 10-5 dilutions.
  4. dTendrils were formed at the 10-3 to 10-5 dilutions.