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Figure 1 | BMC Microbiology

Figure 1

From: SVARAP and aSVARAP: simple tools for quantitative analysis of nucleotide and amino acid variability and primer selection for clinical microbiology

Figure 1

Nucleotide variability per sliding window of 25 nucleotides in groESL sequences of eleven VGS reference strains. Arrows indicate positions chosen using SVARAP for primer sequence design. R1 and R2 are the two discriminant regions bordered by conserved areas revealed using SVARAP. SVARAPfwd1 and SVARAPrev1 are the primers designed for R1 amplification and sequencing; SVARAPfwd2 and SVARAPrev2 are the primers designed for R2 amplification and sequencing.

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BMC Microbiology

ISSN: 1471-2180

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