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Table 2 Effect of exogenous supplemented pyrimidines on growth of ΔargR strains. Overnight cultures were grown in minimal media with exogenously-supplemented L-arginine (230 μM). Cultures were washed in arginine-free minimal media and replica-plated onto minimal plates supplemented with exogenous cytosine, thymine and uracil. Growth was scored after overnight incubation at 37°C. "-" no growth on the plates after 24 hours incubation

From: Stationary phase expression of the arginine biosynthetic operon argCBH in Escherichia coli

Strain

Genotype

Media

  

LB

M9 + G

M9 + G + P

HS1066

WT

+

+

+

HS1066p

ΔrpoS

+

+

+

HS2404

ΔargR

+

-

+

HS2405

ΔrpoS ΔargR

+

-

+

  1. G, Glucose
  2. P, Pyrimidine- Cytosine, Uracil and Thymine added at 20 μg per ml