Table 2 Primers and probes for real-time PCR
From: Effect of iron on the expression of sirR and sitABC in biofilm-associated Staphylococcus epidermidis
Gene | Forward primer 5'-...-3' | probe: labeled FAM-5'and 3'-TAMRA | Reverse primer 5'-...-3' |
|---|---|---|---|
gmk | AAGGTGCTAAGCAAGTAAGAAAGAAATT | ATGCGTTGTTCATATTTTTAGCGCCTCCA | CAACAAGACGTTCTTTCAAGTCATCT |
sirR | AATATCAAACCACCATCAGTAAGTGAA | AGGGCGTTTAGAAAAGGAAGGCTATGTTGAA | ACGGGCACCTTTATAATGTTTTGT |
sitC | CATTAACAGATGCTGACGTTGTATTTT | AACTGGAAATGGTTGGTTTGAAAAAGCACTTG | CCTTCCTCACCATTTAAAGTATATTGG |
sitB | TTTGTCATGTTATTACTCGCACTTGTA | CTGTTGCGAGCATACAAACAGTTGGAATTATCC | AGCTGTAGAAGCTGGAGTGATTAGTAAA |
sitA | TGCAGAATTAAGTGGTGGACAATT | AACGTGTTCTTGTTGCAAGAGCACTTATGAGTAATAGT | CCAACAAAAGGTTCGTCTAAACAAT |