Figure 2

Fungal type I cellulose binding domain (CBD) is widespread in oomycetes. (A) Multiple sequence alignment of 18 oomycete type I CBDs with the domain of Trichoderma resei Cel6A (Tr_Cel6A). The four conserved cysteine residues are marked with asterisks. The glutamine and three aromatic residues that are known to be important for binding the carbohydrate substrate are shown by arrows. Sequence names refer to the Saprolegnia parasitica unigene (Sp_), Phytophthora infestans (Pi_), Phytophthora sojae (Ps_), and Phytophthora ramorum (Pr_) followed by the OGD accession number, GenBank accession number, or NCBI Trace Archive identifier (Ti number). Multiple domains originating from the same sequence are marked with the letters a or b at the end of the sequence name. (B) Consensus sequence pattern of the oomycete type I CBD. Consensus sequence was calculated using WebLogo. The bigger the letter, the more conserved the amino acid site. The positions of amino acids in the consensus sequence correspond to the positions in the sequence alignment in panel A.