Table 4 Primers for PCR

gbs0001

sag0001

-

5'-gtagctgatagtcctggc-3' and 5'-agtccccaactaaagcgc-3'

gbs0045-46

sag0046

MME

5'-aaatgggacacgtacgg-3' and 5'-attgccgccatctcaggg-3'

gbs0217

gbs0227

sag0224

sag0234

I

5'-caagcctttaatgctcgc-3' and 5'-aactgaaattccaatcgcc-3'

5'-tcatcgcgaaaatatggag-3' and 5'-cggtcttttagaaactgtgtcc-3'

gbs0247

sag0254

II

5'-gacttatttcaagtttatgg-3' and 5'-acccttatatacgacagc-3'

gbs0367

gbs0388

gbs0393

-

pNEM316-1 (islands III, VII and VIII)

5'-atcgatttaggattcatgcc-3' and 5'-caacattcgcaaaataagcc-3'

5'-cctagatggcgtagaggcag-3' and 5'-ttgctcacagaccataagcg-3'

5'-tcacccctgagacgtttacc-3' and 5'-gatcgtaaccacggtttgct-3'

gbs0467

sag0430

IV

5'-attgatagatcttacttgcg-3' and 5'-tgatgcaatagctattggc-3'

gbs0589

gbs0598

sag0610

sag0617

V

5'-cagggtgttcaaggctacc-3' and 5'-caagcttacgcacccaag-3'

5'-ctttcctaaaacatatttgg-3' and 5'-atatggtaaaaacttaaggc-3'

gbs0628

gbs0660

sag0645

sag0685

VI

5'-tagctcagtttgcgactgg-3' and 5'-ccaacttttgcatctgctg-3'

5'-aattcttgattgatgagcg-3' and 5'-tcagctttaatcaattccc-3'

-

sag0915

Tn916

5'-aagaccaaaagtggcgaac-3' and 5'-gcctttggattcattcctg-3'

gbs1050

gbs1073

sag1015

sag1038

IX

5'-agcagttacttgatttgcc-3' and 5'-tcctgaattagctagtcgc-3'

5'-tctgcttgagataactccc-3' and 5'-caatagcagttatcaaaggg-3'

gbs1120

gbs1125

gbs1135

gbs1143

gbs1145

-

X

5'-cctagatggcgtagaggcag-3' and 5'-ttgctcacagaccataagc-3'

5'-tcgacgtgttttacggttg-3' and 5'-accgaagagatgatgacgac-3'

5'-gggccacactagaaactgc-3' and 5'-aaatccttcatcgctcctg-3'

5'-tcacccctgagacgttacc-3' and 5'-gatcgtaaccacggtttgct-3'

5'-tctctcggcgttattgtcc-3' and 5'-acaaaagcacaagcgactg-3'

gbs1306

gbs1313

sag1233

sag1246

XII

5'-ctttactggcttcacttgg-3' and 5'-gttgatacaggcattgagc-3'

5'-gattactctaccagtgagg-3' and 5'-agaatagtctgcttcaccc-3'

gbs1987

gbs2008

sag2029

sag2053

XIII

5'-ctgacaattgctttgtttcg-3' and 5'-ggctaacccaaatgtaccg-3'

5'-gctcctctgattaatgccc-3' and 5'-caagctcttgttcggttgc-3'

gbs2082

sag2123

XIV

5'-tttctgggaaaaatcagtgg-3' and 5'-ttttcccgaacaaatgatg-3'