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Table 2 Theoretical patterns of restriction digestion of short 16S-23S intergenic spacer in different species of Bacteroides, Bifidobacterium, Carnobacterium, Enterococcus, Escherichia, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Propionibacterium, Staphylococcus, Streptococcus, and Weissella

From: Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling

 

Sph I

Nco I

Nhe I

Ssp I

Sfu I

Eco RV

Dra I

Vsp I

Hinc II

Eco RI

Avr II

Hind III

Bact. fragilis

-

-

-

-

+

-

-

-

+

-

-

-

B. longum

-

-

-

-

+

-

-

-

-

+

-

-

B. lactis

-

-

-

-

-

-

-

-

-

-

-

-

C. piscicola

-

-

-

+

-

-

++a

-

-

-

-

+

C. gallinarum

-

-

-

-

-

-

++

-

-

+

-

+

C. mobile

-

-

-

-

-

-

-

-

-

+

-

-

Ent. faecalis

-

-

-

+

-

-

-

-

-

-

-

-

Ent. faecium

-

-

-

+

-

-

-

-

-

-

-

-

Ent. raffinosus

-

-

-

+

-

-

-

-

-

-

-

+

E. coli

-

+

-

-

+

-

+

-

-

-

+

-

L. lactis

-

-

-

-

-

-

+

-

-

-

+

-

Leuc. mesenteroides

-

-

+

-

-

-

++

-

-

-

-

+

O. oeni

-

-

-

-

+

-

+

-

-

-

-

+

Ped. acidilacitii

+

-

+

-

-

-

+

++

-

-

-

+

Ped. parvulus

+

-

+

-

-

-

+

-

-

-

-

+

P. freudenreichii

-

-

-

-

-

-

-

-

-

-

+

-

Staph. aureus

+

-

+

+

+

-

+++

+

+

+

-

++

Strep. thermophilus

-

-

-

+

-

-

-

-

-

-

-

-

W. confusa

-

-

-

+

-

-

+++

-

-

-

-

+

W. paramesenteroides

-

-

-

-

-

-

+++

-

-

-

-

-

  1. aTwo or more restriction sites found into spacer region