Figure 3From: 16S rRNA gene based analysis of Enterobacter sakazakii strains from different sources and development of a PCR assay for identificationDefining the detection limit of the PCR system. Decreasing amounts of purified E. sakazakii strain ATCC 51329 genomic DNA target (100 ng – 1 pg) were amplified by PCR. Lane 1, DNA 100 bp marker; In lanes 2 to 5, 1 ng, 100 pg, 10 pg, 1 pg of E. sakazakii DNA was used per reaction.Back to article page