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Table 2 PCR primer sequences

From: Brucella 'HOOF-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs)

Primer name

5' to 3' sequence

Reverse Primer

LOCUS-1 Fwd

HEX·GGT GAT TGC CGC GTG GTT CCG TTG AAT GAG

REV-3

LOCUS-2 Fwd

HEX·CCC GCA TGA TCC GCG AAC AGC TGG ATG

REV-1

LOCUS-3 Fwd

NED·CAG GCG CTT GAG GAT GAG GCG GCA G

REV-3

LOCUS-4 Fwd

6-FAM·GCA GAA TTT TCG AGG CAT TCG GCG ATG

REV-3

LOCUS-5 Fwd

6-FAM·GTG CTC CAG GGC GCC GGG AGG TAT GTT TAG

REV-3

LOCUS-6 Fwd

NED·GCC GCA GGA AAG CAG GCG ATC TGG AGA TTA TC

REV-3

LOCUS-7 Fwd

6-FAM·CAG AGC CGT CGG TGG TTA CTT GAG TAG GGC AG

REV-1

LOCUS-8 Fwd

NED·GTG GGA AGC GTT ATC CTT TAA CGG GAG TAA GGG

REV-1

REV-1

GGG GAG TAT GTT TTG GTT GCG CAT GAC CGC

--

REV-3

GGG GGC ART ARG GCA GTA TGT TAA GGG AAT AGG G

--

  1. HEX, NED and 6-FAM are fluorescent dyes covalently bound to the 5' end of the synthetic primer.