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Table 2 Two hybrid analysis of 'radical' site-directed mutants of LcrG (residues 7–40) with LcrV.

From: Interaction of the Yersinia pestis type III regulatory proteins LcrG and LcrV occurs at a hydrophobic interface

Mutation

Filter-lift reaction

β-galactosidase activitya

wildtype

+

68.05 ± 2.96

LcrG aloneb

-

6.71 ± 0.51

Y9R

+

83.09 ± 5.12

L13E

+

55.10 ± 4.31

A16R

-

4.45 ± 0.42

A16D

-

4.07 ± 0.22

L18R

+

66.03 ± 4.94

A19R

+

86.61 ± 8.92

I20R

+

36.44 ± 3.72

S23R

+

14.30 ± 1.61

A27E

+

21.99 ± 1.24

L30T

+

53.72 ± 3.54

L30R

-

1.91 ± 0.26

M33R

+

52.42 ± 2.10

I37R

+

35.66 ± 2.38

  1. aβ-galactosidase activity is expressed as Miller Units ± standard error of the mean. bThis value is derived from yeast expressing LcrG and an empty vector control.
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BMC Microbiology

ISSN: 1471-2180

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