Strains or plasmids | Characteristics | Source or reference |
---|---|---|
Strains | Â | Â |
E. coli DH5α | F-, Ф80d lacZ∆M15, ∆(lacZYA-argF)U169, endA 1, recA 1, hsdR 17(rk-, mk+) deoR, thi-1, supE 44, λ-, gyrA 96(Nalr), relA 1 | Invitrogen |
E. coli SM10(λ pir) | thi thr leu tonA lacY supE recA::RP4-2-TC::Mu Km λpir | [23] |
L. hongkongensis HLHK1 to HLHK30 | Thirty human strains isolated from patients with community-acquired gastroenteritis in Hong Kong | [3] |
HLHK9 | HLHK9 derivative with Sm resistance phenotype, Sm+ | This study |
HLHK9∆ureA | HLHK9 derivative with ureA deletion, Sm+ | This study |
HLHK9∆ureC | HLHK9 derivative with ureC deletion, Sm+ | This study |
HLHK9∆ureD | HLHK9 derivative with ureD deletion, Sm+ | This study |
HLHK9∆ureE | HLHK9 derivative with ureE deletion, Sm+ | This study |
HLHK9∆arcA1 | HLHK9 derivative with arcA1 deletion, Sm+ | This study |
HLHK9∆arcA2 | HLHK9 derivative with arcA2 deletion, Sm+ | This study |
HLHK9∆arcA1/arcA2 | HLHK9 derivative with arcA1 and arcA2 double deletion, Sm+ | This study |
HLHK9∆ureA/arcA1/arcA2 | HLHK9 derivative with ureA, arcA1 and arcA2 triple deletion, Sm+ | This study |
Plasmids | Â | Â |
pUC19 | Cloning vector; ori lacZ Ampr | Invitrogen |
pCVD442 | Suicide plasmid; R6K ori mob RP4 bla sacB | [25] |
pDS132 | Suicide plasmid; R6K ori mob RP4 cat sacB | [22] |
pDS132-ureA | pDS132 carrying 5′- and 3′-flanking regions of ureA for mutagenesis of ureA | This study |