Figure 2From: The Unculturables: targeted isolation of bacterial species associated with canine periodontal health or disease from dental plaque A simplified example of the pooling strategy used to screen multiple mixed species colonies with the minimum number of qPCRs. White arrows show how colonies were pooled by columns and by rows. If the mixed colony circled in red contains a target species a lower cQ will be observed in qPCR reactions from pools A and 2.Back to article page