Figure 3From: Characterization and regulation of the Resistance-Nodulation-Cell Division-type multidrug efflux pumps MdtABC and MdtUVW from the fire blight pathogen Erwinia amylovora Virulence assay on apple rootstock MM106. Bacteria were inoculated by prick technique in the shoot tips with an inoculum of 5 × 106 CFU/shoot. Establishment of a population of E. amylovora Ea1189 wild type (○), mdtUVW-deficient mutant (●) and mdtABC-deficient mutant (◊) was determined 1, 3 and 7 days post inoculation (dpi), respectively. A minimum of 5 plant shoot tips were inoculated. Data points represent the CFU/cm stem per shoot tip. The horizontal line through the points of a dataset indicates the mean value of the replicates. Average values and standard deviations are listed in Additional file 7.Back to article page