Whole-genome sequencing reveals novel insights into sulfur oxidation in the extremophile Acidithiobacillus thiooxidans

Table 1 Primers used in qRT-PCR detection of genes related to sulfur oxidation

Primer		Sequence (5′ to 3′)	Product size/bp	Anneal temp./°C
Name	Orientation
sqr	Forward	GCTCGGCAGCCTCAATAC	136	56
	Reverse	GGTCGGACGGTGGTTACTG
sor	Forward	AAGCCCGTGCCTAAAGTG	266	56
	Reverse	CTGCCATAGTTGGTGTTGT
doxD	Forward	CATCCCAGGACTCCACAA	223	56
	Reverse	GTCGCCACCTATTCTTACTATC
tetH	Forward	TGAAAGACACGCTACCCG	270	56
	Reverse	GGCCGCTCAATGATAACC
hdrA	Forward	CCGATTTGAAGGTGAAGC	185	56
	Reverse	CGGTTGCGACCATCTGTT
hdrB	Forward	GTGGACCAGCGGGAAGAA	126	56.5
	Reverse	TACCACGGCTCTGGCATCG
hdrC	Forward	TATTGAGTTTGGTCGCATTG	114	55.5
	Reverse	CCCTTGGACAGACGCTTT
soxA-I	Forward	GCTCAGTCAGGGTAAGGC	161	56
	Reverse	GACAACTATTCAAACGCATC
soxB-I	Forward	GCGTATTACCGATTTGCG	198	56
	Reverse	GGATTACCGGCCATGTTT
soxX-I	Forward	GCAGGGTAATTGTTTGGC	163	56
	Reverse	CATATTGATGTGCGGGAT
soxY-I	Forward	GGAATGTCAGCAGTGGGTAT	203	56
	Reverse	TTCTCCGCTATGGTTGGT
soxZ-I	Forward	AAGCGGGCAAGTTGATTC	173	56
	Reverse	CGTATTGTCTTTCCAGGTC
soxA-II	Forward	ATCTTGATGCCGTTGCTG	164	56
	Reverse	GCCCATTTCCCGACTTAT
soxB-II	Forward	CCGTAAGGCATCACAGAG	244	56
	Reverse	CAAGGTATTAGCCCGTTT
soxX-II	Forward	CACAAATAGTCGGCAACCT	237	56
	Reverse	CGCTCAGGGAAACTGTCTT
soxY-II	Forward	TGATGCGTTGTTGGATGT	180	56
	Reverse	CGCCCACTATTGCTGAAAA
soxZ-II	Forward	AGGTAGGGATTGGCACTG	120	56.5
	Reverse	CAAAGATAAGGCTGGAAAA
rhd	Forward	GTGGTCCTGCTTACCCTCAA	130	56
	Reverse	GCCCGATAATATCCTGCTACTG
gapdh	Forward	TAGCCCAGAACGCCTTTG	141	56
	Reverse	CGGTATGTCTTTCCGAGTG

ISSN: 1471-2180