Skip to main content
Figure 1 | BMC Microbiology

Figure 1

From: Colony-live — a high-throughput method for measuring microbial colony growth kinetics— reveals diverse growth effects of gene knockouts in Escherichia coli

Figure 1

An overview of the Colony-live system. (A) Scanners in incubator. (B) Each scanned image contains four plates in which 1536 colonies were grown. (C) Example of image analysis. (Upper) Raw and analyzed images of a growing colony at 9 different incubation time points (2.5-h intervals; from 0 h to 20 h). Colony growth was quantified by integrating the mass of the whole (mass) or the center (mass*) region of a colony. Colors represent pixel absorbance values. (Lower) A cross section view of a growing colony at 9 time points corresponding to the upper images. The mass* region is indicated. (D) Example of growth analysis. Time-dependent mass* values (dots) of a growing colony were regressed to the Gompertz model (black line) and summarized into lag time of growth (LTG), maximum growth rate (MGR), and saturation point of growth (SPG). This example is an ackA mutant showing longer LTG for better display of LTG.

Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us