Figure 1

Metal tolerances of different P. putida strains. P. putida wild-type strain PaW85 (wt), the colS-deficient strain (colS), colS-deficient strain complemented with the colS gene under the control of the inducible P_tac promoter (StacS), colR-deficient strain (colR), colR-deficient strain complemented with the colR gene under the control of the inducible P_tac promoter (RtacR) and colR-deficient strain complemented with the D51A mutant colR gene under the control of the inducible P_tac promoter (RtacR_D51A) were grown on solid LB medium containing different metal salts for 20 hours at 30°C. ColS and ColR expression was induced with 0.5 mM IPTG indicated by “+”. Approximately 5000 cells were inoculated per spot.