Table 3 Primers used in this study
Primers | Target | Sequences (5’ → 3’) |
|---|---|---|
EmF | cesB | GACAAGAGAAATTTCTACGAGCAAGTACAAT |
EmR | Â | GCAGCCTTCCAATTACTCCTTCTGCCACAGT |
14 F | pXO1-14 | GGTAAAGAGTGCGGAAAATGA |
14R | Â | AATACGCCAACGCCAACTTA |
45 F | pXO1-45 | TGCAGCTCGTAATCCACAG |
45R | Â | TGCTAATGATAAAACGCCTGG |
50 F | pXO1-50 | TTCGTACAGATGAAACACAGG |
50R | Â | GTGCCTCAAGATGAACCTTC |
55 F | pXO1-55 | GATAGAGACTGCTCTTGGGAA |
55R | Â | GGTCTTAGCCATGAGAGTAAAAACA |
58 F | pXO1-58 | TGTGATGGACCTTTGTATTAATTTGT |
58R | Â | ATACCCCGCATGGAGCTTAG |
ISF_Sac I | ISces | GCAGAGCTC GGTTCTGGTGCAAAAACTTCAGGACA |
ISR_Xba I | Â | GCATCTAGA GGTTCTGGTGCAAAAAGATAATAAAG |
ISF_Hin dIII | ISces | GCAAAGCTT GGTTCTGGTGCAAAAACTTCAGGACA |
ISR_Bam HI | Â | GCAGGATCC GGTTCTGGTGCAAAAAGATAATAAAG |
KmF_Xba I | Km | TCATCTAGA TAAACCCAGCGAACCATTTG |
KmR_Bam HI | Â | TCAGGATCC TCTAGGTACTAAAACAATTCATCCAG |
ISF3 | ISces | TCTGGTGCAAAAACTTCAGG |
ISR3 | Â | AAGTCGCATACGACCAGGTA |
kmF3 | Km | GAGGATGAGGAGGCAGATTG |
KmR3 | Â | CGGCCAGATCGTTATTCAGT |
APF1 | bla | TTTGCCTTCCTGTTTTTGCT |
APR1 | Â | TTGCCGGGAAGCTAGAGTAA |
ISL-SP1 | CTTCATCCTCTTCGTCTTGGTAGC | |
ISL-SP2 | GGTTCGCTGGGTTTATCTAGAGGT | |
ISL-SP3 | GACAGACTGGTCCCGTAAATCAAC | |
ISR-SP1 | ATATCGGGGAAGAACAGTATGTCG | |
ISR-SP2 | GTACCTAGAGGATCCGGTTCTGGT | |
ISR-SP3 | GACAGACTGGTCCCGTAAATCAAC | |
IS-LR | CTTTCGAATCAACAGCACGA | |
CesD-SP1 | GGCCTATTGTATAATGACAACG | |
CesD-SP2 | GGTGTATTATTTATCTTCGCCTG | |
CesD-SP3 | GGTATTTTAGGGGCGAAGGTTC | |
MH-SP1 | CACTCTTGCGTTTTTGCGTATC | |
MH-SP2 | AAACAATGAGCCCACCCCGAAA | |
MH-SP3 | CGCTTTTCCACATTCTTTACGG | |