Figure 2

5’ or 3’ end of the S. pneumoniae MLST typing region that is not obtained by both the forward and reverse standard primers aligned with the sequencing results from both the forward and reverse alternative primers. Panels (A) through (F) depict the sequencing results of the alternative primers in relation to their corresponding typing region. The angle bracket and top sequence identify the 5’ or 3’ end of the typing region, the middle sequence is the result from sequencing with the forward alternative primer, and the bottom sequence is the result from sequencing with the reverse alternative primer.