Figure 4From: Characterization of AcrD, a Resistance-Nodulation-Cell Division-type multidrug efflux pump from the fire blight pathogen Erwinia amylovora Electrophoretic mobility shift analysis of BaeR interaction with Cy5-labeled DNA fragments. DNA fragments contain the promoter regions of (A) acrD (246Â bp), (B) acrA (205Â bp) and (C) tolC (291Â bp), respectively. Approximately 0.16 pmol of the DNA fragments were incubated with increasing amounts of His-tag purified BaeR (indicated at the top of each lane). The DNA-protein complexes were separated on 4% non-denaturing polyacrylamide gels.Back to article page