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Figure 1 | BMC Microbiology

Figure 1

From: More than fishing in the dark: PCR of a dispersed sequence produces simple but ultrasensitive Wolbachia detection

Figure 1

Schematic presentation of ARM. (A) Position of ARM in association with VNTR-105 locus plus flanking regions in the w Mel genome (GenBank NC_002978). Scheme for VNTR-105 repeat region was adapted from [13] (see this publication for detailed description of VNTR-105 structural features). Black arrows indicate the full 105 bp core repeat segment. Dashed box represents a disrupted segment. ARM (highlighted in yellow) is located within the intergenic region containing the VNTR-105 repeat region. ARM plus repeat region are flanked by WD_1129 (red; NADH-ubiquinone oxidoreductase, putative) on the 5’-prime end and WD_1131 (green; conserved hypothetical protein, degenerate) on the 3’-prime end. (B) Detailed scheme of ARM. The 315 bp PCR amplicon is generated by primer ARM-F (21-mer) and ARM-R (18-mer). Both primers are displayed above and below the PCR amplicon (indicated in yellow).

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