DNA-binding domain | Activation domain | Relative β-gal activity |
---|---|---|
VipB | None | 0.5 ± 0.1% *** |
VipB | VipA | 100.0 ± 5.8% |
VipB | VipA Δ104-113 | 1.0 ± 0.2% *** |
VipB | VipA D104A | 92.7 ± 4.1% |
VipB | VipA V106A | 92.4 ± 3.4% * |
VipB | VipA V110A | 74.6 ± 3.4% *** |
VipB | VipA D104A/V106A | 64.1 ± 10.7% * |
VipB | VipA V110A/L113A | 1.1 ± 0.3% *** |
VipB | VipA D104A/V106A/V110A | 48.8 ± 2.0% *** |
VipB | VipA D104A/V106A/V110A/L113A | 1.0 ± 0.2% *** |