Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

Table 4 Efficiency of the Lactobacillus spp. and G. vaginalis detection in adhesion assays with HeLa cell line

Concentration of cells (CFU/ml)		Multiplex PNA-FISH assay
L. crispatus	G. vaginalis 5-1	Lac663 Probe efficiency	Gard162 Probe efficiency
1×10⁹	1×10⁹	+++	+++
1×10⁵	1×10⁵	+++	+++
1×10³	1×10³	++++	+++
*L. iners*	*G. vaginalis 5-1*	Lac663 Probe efficiency	Gard162 Probe efficiency
1×10⁹	1×10⁹	+++	+++
1×10⁵	1×10⁵	+++	+++
1×10³	1×10³	++	+++

The PNA probe (Lac663 and Gar162) efficiencies were tested in each sample with the following hybridization PNA FISH qualitative evaluation: (++) Moderate hybridization; (+++) Good hybridization; (++++) Optimal hybridization. The table shows the median value from the three experiments for each sample.

ISSN: 1471-2180