| | K-MAP | K-MAP + L-NP-51 | L-MAP | L-MAP + L-NP-51 |
|---|
Day 90
|
3/4
|
3/4
|
4/4
|
4/4
|
Day 135
|
2/2*
|
3/4
|
4/4
|
3/4
|
Day 180
|
3/4
|
2/4
|
2/4
|
3/4
|
- Tissues were stained with Hemotoxylin & Eosin (H & E stain) prior to evaluation. For K-MAP samples at Day 135, only two sets of animal tissues were available for examination due to early expiration of animals before the harvest date (these data are highlighted with ‘*’). Control animals did not demonstrate granuloma formation at Day 90 and Day 180; Day 135 control animals were contaminated and were positive for granulomas in liver tissues. The data represent the number of animals that demonstrated granuloma formations per total animals examined (n =4). Experimental groups included are the following: animals fed normal chow and infected with viable MAP cells (Live-MAP; L-MAP); animals fed viable probiotics in chow and uninfected (Live NP-51; L-NP-51); animals fed viable probiotics in chow and infected with non-viable MAP cells (K-MAP + L-NP-51); animals fed viable probiotics in chow and infected with viable MAP cells (L-MAP + L-NP-51). These data demonstrate MAP infection of tissues regardless of viable or non-viable NP-51 consumption. Additionally, these data evidence that host tissues produce granulomas from exposure to K-MAP antigens.
