Figure 5
From: Homeostatic properties of Lactobacillus jensenii engineered as a live vaginal anti-HIV microbicide
Absence of a pro-inflammatory cytokine response in L. jensenii colonized epithelial model. (Figure 5a) TNF-α, (Figure 5b) IL-6, (Figure 5c) IL-1α, (Figure 5d) IL-1RA cytokine levels measured in supernatants from vaginal (Vk2/E6E7) epithelium cultured for 24 h with L. jensenii 1153–1666, 3666, and gfp bioengineered strains and L. jensenii 1153 wild (WT) strain or MALP-2 (50 nM) as a positive control. Bars represent mean and SEM from duplicate and triplicate cultures in two independent experiments. *** P<0.001,* P<0.05 different from medium control, +++P<0.001 different from L. jensenii 1153 WT.