Figure 5

Enrichment of genomic DNA using the α-La1 scFv significantly improves genome coverage and amplification bias. A single cell per well, or 50 cells per well were sorted from gate P3 and sequenced using Illumina MiSeq. A) Sequencing reads mapped to L. acidophilus NCFM shows significantly more complete coverage (99.8%) when using the 50-cell template versus a single cell template. B) De novo assembled contigs mapped back to the reference sequence show essentially complete coverage (>99.8%) with far less amplification bias.