Table 6 List of primers used in this study for PCR amplification and sequencing of the four CRISPR-MVLST markers
Primer | Orientation | Primer sequence (5′-3′) | Annealing temp. | PCR | Sequencing |
|---|---|---|---|---|---|
CRISPR1-5 | Forward | TGAAAACAGACGTATTCCGGTAGATT | 55.5 | ✓ | ✓ |
CRISPR1-1 | Reverse | CAGCATATTGACAAGGCGCT | ✓ | ✓ | |
CRISPR2-3 | Forward | ATTGTTGCGATTATGTTGGT | 57 | ✓ | ✓ |
CRISPR2-1 | Reverse | TCCAGCTCCCTTATGATTTT | ✓ | ||
CRISPR2-4 | Reverse | GCAATACCCTGATCCTTAACGCCA | ✓ | ||
CRISPR2-5 | Reverse | CGACGAAATTAAAACCGAACT | ✓ | ||
CRISPR2-6 | Forward | CGGATTCCATGCGTTTTCA | ✓ | ||
CRISPR2-7 | Forward | CCGGCGAGGTCAATAAAA | ✓ | ||
CRISPR2-8 | Forward | TGACGCTGGTCTATACCG | ✓ | ||
CRISPR2-9 | Forward | GTGACGTCAGTGCCGAA | ✓ | ||
CRISPR2-10 | Reverse | CTCTTCGCACTCTCGATCAA | ✓ | ||
fimH-1 | Forward | AGGTGAACTGTTCATCCAGTGG | 56.7 | ✓ | ✓ |
fimH-2 | Reverse | GCGGGCTGAACAAAACACAA | ✓ | ✓ | |
sseL-1 | Forward | AAAATCAGGTCTATGCCTGATTTAATATATC | 60 | ✓ | |
sseL-2 | Reverse | GGCTCTAAGTACTCACCATTACT | ✓ | ||
sseL-3 | Forward | ACCAGGAAACAGAGCAAAATGAATATATGT | ✓ | ||
sseL-4 | Forward | TTCTCTCGGTAAACTATCCTATTGGGC | ✓ |