Figure 1

Analysis of variant and ancestral strain biofilm co-cultures. P. fluorescens variants and ancestral strain co-cultures were analyzed by the introduction of different colour AFPs. CLSM images were obtained on 96 h biofilms grown in the CBD. See ‘Materials and Methods’ for details of acquisition parameters. Multiple replicates were obtained for each biofilm co-culture and shown here are the best representative images. The images show a top-view 3D reconstruction of the biofilm along with a cross-section through the y-axis. Scale bars represent 40 μ m. A, Controls showing that the two variants grow evenly together and the wildtype (CHA0) and Δ gacS (CHA19) also grow evenly distributed throughout the biofilm. B, Paired co-cultures of the CHA0 and CHA19 strains with either the SCV or WS. C, Triple co-cultures were done, where the SCV and WS were cultured together with either CHA0 or CHA19.