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Table 1 Sequence of primers used to amplify amastin isoforms ORFs.

From: Distinct genomic organization, mRNA expression and cellular localization of members of two amastin sub-families present in Trypanosoma cruzi

Primer name / gene ID

Primer Sequence (5’-3’)

Restriction enzyme

pδ1-amastin (F) Tc00.1047053511071.40

TTGTTCTAGAGTAGGAAGCAATG

XbaI

pδ1-amastin (R) Tc00.1047053511071.40

CGCTGGATCCGAACCACGTGCA

BamHI

β1-amastin (F) Tc00.1047053509965.390

CCTAGGAGGATGTCGAAGAAGAAG

AvrII

β1-amastin (R) Tc00.1047053509965.390

AGATCTCGAGCACAATGAGGCCCAG

BglII

β2-amastin (F) Tc00.1047053509965.394

TCTAGATGGGCTTCGAAACGCTTGC

XbaI

β2-amastin (R) Tc00.1047053509965.394

GGATCCCCAGTGCCAGCAAGAAGACTG

BamHI

  1. The underlined sequences correspond to the restriction sites recognized by the restriction enzyme.
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BMC Microbiology

ISSN: 1471-2180

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