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Table 1 Gene locus, given names, features, gene conservation, sequence of the primers employed for DNA amplification, and molecular mass of expressed recombinant proteins

From: “Features of two proteins of Leptospira interrogans with potential role in host-pathogen interactions”

Gene locus1

Given name2

Description/

Function

Conservation (identity)3

Sequence of primers for PCR amplification

Molecular mass

LIC11834

Lsa33

Putative lipoprotein

Lai (99%)

LBH (87%)

LBP (31%)

F:5′CTCGAG GATCTACAAGGTGGGGTTTTTAC3′

XhoI

R:5′CCATGG TTACTGAGGTTTTACTTGGTCC3′

NcoI

33.1 kDa

LIC12253

Lsa25

Conserved hypothetical protein

Lai (100%)

LBH (77%)

LBP (39%)

F:5′ CTCGAG GAGGAGAAACCGGACGATAC 3′

XhoI

R:5′CCATGG TTAGGGAAGACTTCTAACACATC3′

NcoI

24.07 kDa

  1. 1http://aeg.lbi.ic.unicamp.br/world/lic/; LIC: Leptospira interrogans Copenhageni
  2. 2Lsa: Leptospiral surface adhesin of 33 and 24 kDa; we have named the latter as Lsa25 because Lsa24 has been already described (Barbosa et al., 2006)
  3. 3 http://blast.ncbi.nlm.nih.gov/Blast.cgi/