Table 1 Characteristics of seven microsatellite markers developed from 'Candidatus Liberibacter asiaticus'
SSR Markers | Primer sequences (5'-----3') | Repeats | Location in genome | ORF | Ta (°C) | Size range (bp) | No. of alleles | H |
|---|---|---|---|---|---|---|---|---|
LasSSR-A-f LasSSR-A-r | FAM-CGCCTACAGGAATTTCGTTACG TCTCATCTTGTTGCTTCGTTTATCC | (TATTCTG)8 | 255477-255753 | adenosine deaminases | 50°C | 241-434 | 30 | 0.881 |
LasSSR-B-f LasSSR-B-r | VIC-ATCGCCTATAAATCCCTTTACTGATATGTTTCC TGGTAACGGAAGTGATAATAACTACAGCAATAAG | (TTTAA)6 | 669257-669458 | hypothetical protein | 60°C | 196-206 | 3 | 0.216 |
LasSSR-C-f LasSSR-C-r | VIC-CGATTGTTGATGAATTACC GAATAGAAGAACCCTAAGC | (CAGT)8 | 666722-666947 | phosphohydrolases | 50°C | 208-254 | 15 | 0.613 |
LasSSR-D-f LasSSR-D-r | NED-CGGTGTCGGTATCGGTATCATTC CGAAGAAGAGACGGAGGTTAAGC | (TTC)5 | 377678-377850 | hypothetical protein | 55°C | 158-174 | 3 | 0.391 |
LasSSR-E-f LasSSR-E-r | NED-GATCAGTAGTCTATCACCAC TACTGGAAACAAATGGAATAC | (CTTGTGT)5 | 354424-354613 | transcriptional regulator | 50°C | 173-290 | 17 | 0.587 |
LasSSR-F-f LasSSR-F-r | FAM-TCGTCTTATCGTATATCACTCC TTCACTATTAAAGGATCAAGGC | (TTTACATC)3 | 520542-520307 | repair ATPase | 52°C | 227-235 | 2 | 0.204 |
LasSSR-G-f LasSSR-G-r | FAM-CGGGAGAAATTAAAGATGATGG CGCTGTTAATACATACTTACGC | (TTGTTGGA)2 | 998251-998403 | hypothetical protein | 53°C | 139-152 | 2 | 0.204 |