Table 4 Carotenoid composition of wild-type and cyp61 X . dendrorhous mutant strain (in ppm)
Strains | |||||||||
|---|---|---|---|---|---|---|---|---|---|
UCD 67-385 | 385-cyp61(+/−) | 385-cyp61(−/−) | |||||||
Cultivation time (h) | 24 | 72 | 120 | 24 | 72 | 120 | 24 | 72 | 120 |
Astaxanthin | 52.6±22.3 | 26.3±2.7 | 224.0±42.1 | 89.1±13.4 | 34.9±5.1 | 223.7±8.6 | 126.5±31.0 | 49.8±18.2 | 434.7±56.2 |
Phoenicoxanthin | ND | ND | ND | ND | ND | ND | ND | ND | ND |
Cantaxanthin | ND | ND | 13.4±3.3 | ND | ND | ND | ND | ND | ND |
HO-keto-γ-carotene | ND | 1.0±0.5 | ND | ND | 1.9±0.3 | ND | ND | 2.2±1.3 | ND |
HO-keto-torulene | 2.6±1.1 | 1.1±0.2 | 30.1±6.7 | ND | ND | 35.5±1.0 | ND | ND | 62.1±7.3 |
Keto-γ-carotene | 8.0±4.9 | 2.7±1.4 | 7.8±1.9 | ND | 1.2±0.6 | 9.7±1.0 | ND | 5.7±2.9 | 21.4±7.9 |
HO-echinenone | 1.8±0.6 | 1.2±0.9 | 2.6±0.5 | ND | 2.6±0.5 | 9.2±0.4 | ND | 3.6±1.6 | 15.6±4.4 |
Echinenone | ND | ND | 2.0±0.4 | ND | ND | ND | ND | ND | ND |
Lycopene | 4.0±2.0 | ND | ND | ND | 1.4±0.7 | 1.1±1.0 | ND | 4.3±1.9 | ND |
γ-carotene | ND | 0.2±0.03 | 2.7±0.5 | ND | ND | ND | ND | 0.8±0.4 | ND |
β-carotene | 1.1±0.5 | 0.8±0.3 | 2.7±1.1 | ND | 1.7±1.0 | 6.3±0.8 | ND | 4.8±3.5 | 15.8±9.1 |
Total carotenoids | 70.7±26.9 | 36.1±8.6 | 290.1±53.4 | 89.1±13.4 | 47.6±7.1 | 293.7±9.1 | 126.5±31.0 | 78.2±26.2 | 555.1±75.2 |
Strains | |||||||||
CBS 6938 | CBS - cyp61 (−) | ||||||||
Cultivation time (h) | 24 | 72 | 120 | 24 | 72 | 120 | |||
Astaxanthin | 32.1±11.2 | 202.0±17.7 | 324.2±6.7 | 62.8±5.4 | 313.5±24.1 | 429.3±26.5 | |||
Phoenicoxanthin | 13.7±8.0 | 8.1±2.1 | ND | ND | ND | ND | |||
Cantaxanthin | ND | ND | ND | ND | ND | ND | |||
HO-keto-γ-carotene | 2.9±1.4 | 9.5±0.6 | ND | 2.7±2.0 | ND | 12.2±10.5 | |||
HO-keto-torulene | ND | 20.1±3.6 | 25.6±12.4 | ND | 76.4±8.3 | 72.8±18.0 | |||
Keto-γ-carotene | 9.8±4.6 | 32.8±4.6 | 29.8±0.45 | 7.1±0.8 | 50.2±3.5 | 33.0±2.97 | |||
HO-echinenone | 1.4±0.8 | 21.9±5.2 | 15.7±0.6 | 3.9±0.1 | 24.1±1.6 | 18.8±1.0 | |||
Echinenone | ND | ND | ND | ND | ND | ND | |||
Lycopene | 16.0±1.3 | ND | ND | 11.9±4.9 | 3.2±0.5 | 2.9±0.1 | |||
γ-carotene | 2.4±2.0 | 7.3±1.6 | 7.6±0.5 | ND | 8.8±0.2 | 15.3±1.7 | |||
β-carotene | 0.4±0.2 | 33.2±6.8 | 20.4±0.7 | 1.8±1.2 | 41.8±4.2 | 31.2±1.4 | |||
Total carotenoids | 78.9±21.3 | 347.2±36.9 | 453±11.1 | 91.9±7.44 | 530.3±21.4 | 625.8±22.9 | |||
Strains | |||||||||
AVHN2 | AV2 - cyp61 (−) | ||||||||
Cultivation time (h) | 24 | 72 | 120 | 24 | 72 | 120 | |||
Astaxanthin | 15.2±0.8 | 116.5±7.0 | 131.8±20.6 | 16.3±6.1 | 118.0±59.2 | 143.0±64.8 | |||
Phoenicoxanthin | ND | ND | ND | ND | ND | ND | |||
Cantaxanthin | ND | ND | ND | ND | ND | ND | |||
HO-keto-γ-carotene | ND | 20.0±1.2 | 17.9±2.8 | ND | 25.3±7.8 | 36.8±16.7 | |||
HO-keto-torulene | 0.7±0.4 | 27.0±10.4 | 21.1±2.6 | 1.1±0.9 | 62.8±22.3 | 40.6±9.9 | |||
Keto-γ-carotene | 3.0±1.07 | ND | ND | 1.7±0.7 | 13.1±9.25 | ND | |||
HO-echinenone | 2.1±0.6 | 10.9±5.7 | 9.9±0.9 | ND | 9.3±7.3 | 13.6±2.6 | |||
Echinenone | ND | ND | ND | ND | ND | ND | |||
Lycopene | 1.4±1.0 | ND | ND | ND | 4.0±2.5 | ND | |||
γ-carotene | ND | 0.8±0.1 | ND | ND | 2.2±1.7 | 1.1±0.9 | |||
β-carotene | 1.0±0.5 | 19.7±12.0 | 12.0±2.9 | 1.9±0.9 | 25.4±7.6 | 20.4±4.7 | |||
Total carotenoids | 24.9±2.8 | 195.3±33.7 | 193.4±19.0 | 25.0±6.9 | 274.6±24.1 | 258.6±76.7 | |||