Table 2 Strains and plasmids used in this study

Escherichia coli BW29427

thrB1004 pro thi rpsL hsdS lacZ ΔM15 RP4-1360 Δ(araBAD) 567 ΔdapA1341::[erm pir (wt)]

[47]

Vibrio harveyi BB120

wild type, ATCC BAA-1116 [reclassified as Vibrio campbellii]

[5, 48]

Vibrio harveyi JAF78

ΔluxO-Cam^R

[13]

pLAFRII

cosmid vector, Tet^R

[49]

pBK-miniTn7-gfp3

mini-Tn7 transposon delivery plasmid

[50]

pBAD24

pBR322 ori, Amp^R

[51]

pBAD24gfp

pBAD24 carrying gfpmut3

[52]

pBAD24gfptet^R

pBAD24 carrying gfpmut3, Tet^R

This work

pCA1

pBAD24 carrying P _recA ::gfpmut3, Tet^R

This work

pCA2

pBAD24 carrying P _luxC ::gfpmut3, Tet^R

This work

pCA3

pBAD24 carrying P _vhp ::gfpmut3, Tet^R

This work

pCA4

pBAD24 carrying P _vscP ::gfpmut3, Tet^R

This work

pCA5

pBAD24 carrying P _luxS ::gfpmut3, Tet^R

This work