Acid stress response and protein induction in Campylobacter jejuni isolates with different acid tolerance

BMC Microbiology

Table 3 Induced proteins (% volume intensity) during HCl (pH 5.2) and acetic acid (pH 5.7) exposure in C. jejuni NCTC 11168, C. jejuni 305 and C. jejuni 327 at 37°C in chemically defined broth

				Campylobacter jejunistrains³
Protein/(NCBInr ¹ )	Mw (kDa)	Score ²		NCTC 11168	305	327
Dps (NP282665)	17.4	222	Vol%
p19 (CAA73983)	17.0	255	Vol%
AhpC (NP281525)	22.0	668	Vol%
SodB (NP281379)	25.0	241	Vol%
TrxB (NP281357)	33.5	204	Vol%
Cj0706 (NP281878)	28.0	431	Vol%
MogA (YP_178829)	20.3	318	Vol%
				C HCl Ac	C HCl Ac	C HCl Ac

Dps: Bacterioferritin, p19: 19 kDa periplasmic protein, AhpC: Alkyl hydroperoxide reductase, SodB: Superoxide dismutase (Fe), TrxB: Thioredoxin-disulfide reductase, Cj0706: hypothetical protein, MogA: Molybdenum cofactor biosynthesis protein.
Columns: light grey: control (C), dark grey: HCl stressed cells (HCl), white: Acetic acid stressed cells (Ac).
¹ Identification was based on Mascot MS/MS Ion Search using sequence data from the database NCBInr.
² Mowse Score (Score).
³ The intensity of the induced proteins was estimated by Image MasterTM 2D Platinum and % volume intensity was calculated.
The intensity of the protein spots was analyzed using the Image MasterTM 2D Platinum (version 5.0, Amersham Biosciences, Melanie). Three biological independent replicates was performed and % volume intensity was calculated as: % volume intensity control _{(protein x)} = volume intensity /(volume intensity control + volume intensity HCl + volume intensity acetic acid).

ISSN: 1471-2180