Figure 6From: Identification and recombinant expression of anandamide hydrolyzing enzyme from Dictyostelium discoideumWestern blotting analysis of distribution of HIS-FAAH in membrane fractions of Dictyostelium. Total cellular protein (L) from AX3FAAH cells were fractionated into 13,000xg membrane and cytosol fractions (P1 and S1 respectively) and 100,000xg membrane and cytosolic fractions (P2 and S2 respectively). Described membrane and cytosolic fractions were separated on 10% SDS-PAGE and subjected to Western blotting using anti-HIS antibody. M represents molecular mass standard in kDa.Back to article page