Table 2 Primers used in this study
Primer | Sequence (5'-3') | Target |
|---|---|---|
PRG1-F | GTC GAC CAA AAT TTG GCT GCT TGT TG | Paralog 1 gene cassettes in Vibrio rotiferianus DAT722 |
PRG1-R | CAT CAG AGA TTT TGA GAC ACA ACC CGA GCG ACA ATT TTA AGC | Paralog 1 gene cassettes in Vibrio rotiferianus DAT722 |
PRG5-F | GGC AGA GCA TTA CGC TGA TCA AAG GTC ATA AGT TTT GGT G | Paralog 2 gene cassettes in Vibrio rotiferianus DAT722 |
PRG5-R | GTC GAC CAT GCG CTA CTT CTA TTT ATG C | Paralog 2 gene cassettes in Vibrio rotiferianus DAT722 |
Kan-F | GTT GTG TCT CAA AAT CTC TGA TG | aphA1 in pLOW2 (F) |
Kan-R | TCA GCG TAA TGC TCT GCC | aphA1 in pLOW2 (R) |
VSD5-F | TGA GCT ACC ACA AGC AAG G | Cassette 5 in Vibrio rotiferianus DAT722 (F) |
VSD14-F | AAA GCG GTT ACA TTC GGG | Cassette 5 in Vibrio rotiferianus DAT722 (R) |
VSD25a-F | ACA TAT GTA GAC CCT GTG CG | Cassette 25 in Vibrio rotiferianus DAT722 (F) |
VSD47-F | CAT TTT AAG TCG GCT CTT CC | Cassette 25 in Vibrio rotiferianus DAT722 (R) |
VSD62-R | GTA GGT AAT TTC GGC TTC TCG | Cassette 62 in Vibrio rotiferianus DAT722 (R) |
VSD25b-F | TGC GCA ATA TAT CGC AAG AG | Cassette 25 in Vibrio rotiferianus DAT722 (F) |
VSD25-R | GCC GTC CAT AGT CGT CAT TT | Cassette 25 in Vibrio rotiferianus sp. DAT722 (R) |
B-VSD11-F | TTT TGG ATC CGA ATA GGG AAA ATC CGT G | Gene from cassette 11 in V. rotiferianus DAT722 (F) |
P-VSD11-R | TTT TCT GCA GTT AGT TGA ATT GTT TCA CAG C | Gene from cassette 11 in V. rotiferianus DAT722 (R) |