Strain or plasmid | Relevant genotypea | Reference or source |
---|---|---|
Strains | Â | Â |
V. rotiferianus DAT722 | Â | Â |
DAT722 | Wild-type | [11] |
DAT722-Sm | DAT722; Spontaneous SmR mutant. | This study |
MD7 | DAT722-Sm; Single recombination cross-over of pVSD2 into cassette 61, KmR | This study |
d8-60a | DAT722-Sm; Δcassettes 8-60, SmR, KmR | This study |
d8-60b | DAT722-Sm; Δcassettes 8-60, SmR, KmR | This study |
d8-60b-S | DAT722-Sm; Δcassettes 8-60, SmR, KmR. Spontaneous mutant of d8-60b. | This study |
d8-60c | DAT722-Sm; Δcassettes 8-60, SmR, KmR | This study |
d8-60c-S | DAT722-Sm; Δcassettes 8-60, SmR, KmR. Spontaneous mutant of d8-60c. | This study |
d16-60 | DAT722-Sm; Δcassettes 16-60, SmR, KmR | This study |
E. coli | Â | Â |
XL1-Blue | F' proAB lacIqZΔM15 Tn10/recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relAi, TcR | Stratagene |
SY327 λ pir | Δ(lac pro) argE (Am) rif nalA recA56 | [38] |
SM10 λ pir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu, Tcr KmR | [39] |
Plasmids | Â | Â |
pLOW2 | Cloning vector, KmR | [40] |
pGEM-T Easy | Cloning vector, ApR | Promega |
pMAQ1080 | pGEM-T Easy carrying a 1834-bp fragment. The fragment was created using fusion PCR and consists of, in order, a 448-bp of paralog group 1 sequence, a 964-bp fragment containing aphA1 and a 410-bp paralog group 2 sequence abutted by salI restriction sites. | This study |
pCVD442 | Mobilisable sacB counter-selectable suicide vector, ApR | [41] |
RK600 pJAK16 pMAQ1081 pMAQ1082 | ColE1 oriV; RP4tra+ RP4 oriT; CmR; helper plasmid in triparental matings Low copy IPTG-inducible expression vector, CmR salI fragment from pMAQ1080 cloned into the unique salI site of pCVD442. pJAK16 containing cassette 11 | [42] [43] This study This study |