Figure 2From: Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phagePlaque formation of M13am9 with gp9-HA coat protein. E. coli K38 bearing pMS-g9-HA was mixed with LB top agar containing 1 mM IPTG and poured on an agar plate. After solidification, M13am9 phage was applied and incubated at 37 °C overnight.Back to article page