Characterization of Conserved Combined T and B Cell Epitopes in Leptospira interrogansMajor Outer Membrane Proteins OmpL1 and LipL41

Table 1 Primers for amplifying epitopes of OmpL1 and LipL41

Protein	Location	Primer	Sequence(5'-3')
OmpL1	59-78	O1-F59	cgGGTACCTTTCTATTCTCACTCTgttcgatcgtccaatacctg
		O1-R59	ttCGGCCGagccgcctgggttttgaaaacaagcag
	87-98	O1-F87	cgGGTACCTTTCTATTCTCACTCTtatataggagttgctcctag
		O1-R87	ttCGGCCGagccgccagcaggaatcgcttttctag
	173-191	O1-F173	cgGGTACCTTTCTATTCTCACTCTagttctatcgtcattcctgc
		O1-R173	ttCGGCCGagccgccagcgtcttcagtaacattc
	297-320	O1-F297	cgGGTACCTTTCTATTCTCACTCTctttctccttttccagc
		O1-R297	ttCGGCCGagccgccgagttcgtgtttataaccg
LipL41	30-48	L41-F30	cgGGTACCTTTCTATTCTCACTCTgtattcccgaaagataaaga
		L41-R30	ttCGGCCGagccgccacgaatggttccgaggaat
	181-195	L41-F181	cgGGTACCTTTCTATTCTCACTCTgtacgtatgatgttaattc
		L41-R181	ttCGGCCGagccgcctactttaatgagagtagc
	233-256	L41-F233	cgGGTACCTTTCTATTCTCACTCTgaagctgcttatatc
		L41-R233	ttCGGCCGagccgcctttaacgaaaactttaattc
	263-282	L41-F263	cgGGTACCTTTCTATTCTCACTCTaaagaacttcttcaagaaggtt
		L41-R263	ttCGGCCGagccgccttttttgaaacttggagtttc

EcoR52 I and Kpn I sites are capital and underlined. Sequence encoding M13KE leader peptide is capitalized. The sequences in bold encode flexible peptide.

ISSN: 1471-2180