Table 1 Bacterial strains, plasmids and primers used in this study
From: Roles of RpoN in the resistance of Campylobacter jejuni under various stress conditions
Strains, plasmid and primers | Description | Source |
|---|---|---|
E. coli | Â | Â |
DH5α | F', Φ80 dlacZΔM15, endA1, recA1, hsdR17 (r k -, m k +), supE44, thi1, Δ(lacZYA-argF)U169, deoR, λ- | Invitrogen |
C. jejuni | Â | Â |
81-176 FMB1116 FMB2017 | wild type, clinical isolate rpoN::cat rpoN complementation using pFMBComC-rpoN | [51] In this study In this study |
Plasmids | ||
pUC19 pUC-rpoN pUC-rpoN::cat pUC19-16Sto23S pFMBComC pFMBComC-rpoN | Cloning and suicide vector, Ampr pUC19 carrying rpoN and flanking region pUC19 carrying rpoN::cat pUC19 carrying fragments of genes encoding 16s and 28s rRNAs Kanr cassette cloned into pUC19-16Sto23S pFMB carrying rpoN | NEB In this study In this study In this study In this study In this study |
Primers | ||
rpoN_F rpoN_R catF(SmaI) catR(SmaI) rpoNC_F(XbaI) rpoNC_R(XbaI) | ATGATAAGGGTAAGAATTATTTTGAT AAAAAAATCACGCAAGGGGATAAGTCCTC GTGTTCCTTTCCCGGGTAATTGCG GATAAAAACCCGGGGGAACTAAAGGG TTGCAACCAATctAGACGTGAAAAAG AAGTGTAATGGGTTTtcTAGATTGATAG | |