Target gene | GenBank accession no. | Primer* | Primer sequence | Location |
---|---|---|---|---|
Oligonucleotide primers used for RT real-time PCR | ||||
gyrB | 57636585 | gyrB-F | CTTATATGAGAATCCATCTGTAGG | 1110-1263 |
 |  | gyrB-R | AGAACAATCTGCCAATTTACC |  |
lrgA | 57636056 | lrgA-F | TGGACTTGTACTATTATTTATCGC | 165-309 |
 |  | lrgA-R | AAGGATTGGTAAAGAGTTAATGAC |  |
lytS | 57636054 | lytS-F | CTGTTCAAGATAATGGTCAAGG | 1535-1680 |
 |  | lytS-R | CAGTGCCGATGTTGTTCC |  |
serp0043 | 57636640 | serp0043-F | CAAGCACAAGCGTCTTCATC | 73-236 |
 |  | serp0043-R | ACTCTTTCACCATTATTTGTTTCAG |  |
glpQ | 57637130 | glpQ-F | CCGTTACACTGGGTTTAGC | 41-221 |
 |  | glpQ-R | TTACCACTTACTGAGTCTGATTC |  |
arlR | 57636010 | arlR-F | AGAGAATGATGGAAAGGCAGGT | 90-253 |
 |  | arlR-R | ATGTCTCGCTTTTCGCAGTAAT |  |
atlE | 57637180 | atlE-F | AACAACCACAGAATCAGTCTAATC | 92-237 |
 |  | atlE-R | TTGAACTTGGGTAGGGTCTTG |  |
aae | 57637180 | aae-F | AACAAATTGATAAAGCAACG | 1970-2186 |
 |  | aae-R | GTTGTCTTTCCTTTAGTGTC |  |
aap | 57636451 | aap-F | AATAGAACCTACAACTTCAGAACC | 945-1039 |
 |  | aap-R | TGTTATTGGATGAACTATCAGCAG |  |
icaA | 57636387 | icaA-F | GGTTGTATCAAGCGAAGTC | 556-754 |
 |  | icaA-R | ACATCCAGCATAGAGCAC |  |
saeS | 57636974 | saeS-F | GGTATCGTTCCAGAACTTCAATC | 757-881 |
 |  | saeS-R | ATTTGTTGTGCTAACTCATTTGC |  |
saeR | 57636975 | saeR-F | CTCAAGAACATGACACGATATACG | 245-354 |
 |  | saeR-R | TCTAGCGAGAAGGTTATTAGTACG |  |
saeQ | 57636990 | saeQ-F | GCAAGTTTCTTTGGAGCCTTC | 268-447 |
 |  | saeQ-R | CTTATCTTCACCTCGGTTATTACG |  |
saeP | 57636991 | saeP-F | CTAACTCGGAAAGCGATCAC | 71-258 |
 |  | saeP-R | GTCTGGACCTTTAGAAGATTTG |  |
Oligonucleotide primers used for eDNA quantification | ||||
gyrA | 57636584 | gyrA-F | CCTTATGAAACTCGGAGATGG | 2382-2489 |
 |  | gyrA-R | TCAGTAGTAGTAGATTGTTGCG |  |
lysA | 57637514 | lysA-F | TGACAATGGGAGGTACAAGC | 32-107 |
 |  | lysA-R | TGGTCTTCATCGTAAACAATCG |  |
serp0306 | 57636873 | serp0306-F | ATGCCACATCCACGAAAGA | 203-381 |
 |  | serp0306-R | TGTAACTGACAATGCCCAATC |  |
leuA | 57638228 | leuA-F | GTGAACGGTATTGGTGAAAGAG | 685-762 |
 |  | leuA-R | GTGGTCCTTCCTTACATATAAAGC |  |