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Table 4 Kinetic parameters for the racemization of L- to D- and D- to L-alanine by alanine racemases from Gram-positive bacteria

From: The crystal structure of alanine racemase from Streptococcus pneumoniae, a target for structure-based drug design

 

D to L direction

L to D direction

Enzyme [ref]

K m (mM)

V max (U/mg)a

k cat (min-1)

K m (mM)

V max (U/mg)a

k cat (min-1)

Alr SP [21]

2.1b

87.0b

NR

1.9b

84.8b

NR

Alr GS [76]

2.7c

1400c

NR

4.3c

2550c

NR

Alr SL [77]

0.4c

NR

3800c

0.4c

NR

3300c

Alr BA [36]

2.8b

101b

NR

NR

NR

NR

Alr EF [78]

2.2c

1210c

~2340c

7.8c

3570c

~2340c

  1. aOne unit is defined as the amount of enzyme that catalyzes racemization of 1 μmol of substrate per minute.
  2. bAt 23°C. cAt 37°C. NR: not reported.
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BMC Microbiology

ISSN: 1471-2180

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